Posts with the tag: scattering media
In the last few years, several papers have looked at how it might be possible to use a multimode fibre as an ultra-narrow endoscope (see this post and this post for a bit of background). The most common approach is to use a spatial light modulator to shape the wavefront entering the fibre. If this is done in precisely the right way, interference between light coupled into the different modes of the fibre will result in a focused spot at the far end. By adjusting the input wavefront it’s then possible to scan the spot in two dimensions, allowing point-by-point imaging. Of course, we need to know what wavefronts to use, making it necessary to perform a calibration which requires access to the far end of the fibre. Unfortunately, this calibration is highly dependent on the configuration of the fibre – if the fibre is bent then the calibration changes. This means the technique is only applicable to rigid probes, greatly limiting the scope of potential applications. Now, in a paper published in Nature Photonics, Tomáš Čižmár and colleagues from the University of Dundee have suggested a possible solution to this problem.
Optical microscopy can only penetrate a few hundred microns into thick tissue, a limit imposed by scattering. High resolution imaging requires single-scattering events, so when we have multiple-scattering from particles above and below the focal plane, the resolution and signal to noise ratio quickly degrade. The thicker the tissue (i.e. the deeper the plane of interest) the more the multiple-scattering events dominate over single scattering. Techniques such as optical coherence tomography (OCT) enhance the penetration depth by rejecting multiple-scattered light using what is effectively a time-of-flight measurement. This works because light that has been scattered multiple times will tend to have travelled further than light that has been scattered only once. However, even with this technique, the penetration depth seldom exceeds 1-2 mm, as some multiple-scattered photons will (by chance) have a time of flight close to that of the single scattered photons. As we try to go deeper, these events will begin to dominate again. Now, a group mainly from Korea University in Seoul have suggested an additional method of discriminating between single and multiple-scattered photons, using a technique they call “collective accumulation of single-scattered waves”.
A research team from the University of Twente has found a way to obtain high resolution images of a fluorescent object through a strongly scattering medium. This has been a goal of bioimaging scientists for some time, as it would allow us to use visible light to image much deeper into tissue. Various methods have been suggested, but they generally need some kind of ‘guide-star’ behind the scattering layer. The new approach uses the ‘memory effect’ of speckle to avoid the need for any calibration or guide-star, potentially making it much more applicable to real situations.