Posts related to: Fluorescence

High Speed Multi-photon PLIM

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Phosphorescence lifetime imaging microscopy (PLIM) allows substances or tissues with different phosphorescence lifetimes to be identified with high spatial resolution. PLIM hasn’t found many practical applications so far, but it could be useful as a way of measuring oxygen concentration in tissues. Depth resolved images can be obtained using multi-photon excitation, a technique which ensures that all the signal comes from the focal plane. Unfortunately, relatively long phosphorescent lifetimes make the point-by-point scanning used in multi-photon microscopy very time consuming. Attempts to improve the frame rate using parallel excitation can result in cross-talk between pixels and blurring of the image. Now, a group from Cornell University has devised a way to acquire parallel excitation PLIM images which are free from cross-talk.

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Combined Fluorescence and White Light Laparoscopy

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If we wanted to build a fluorescence imaging system for minimally invasive surgery then there are a few things we would need to consider. We would want it to be simple to implement, reasonably lightweight and, most importantly, compatible with existing laparoscopes. We’d also like to be able to obtain a conventional white light view at the same time as the fluorescence. Researchers at GE have developed a device which meets all of these requirements, and recently published the details in the open access journal Biomedical Optics Express. Their suggested application is to help identify nerves during surgery, but the technique could easily be used for a range of purposes.

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