Posts from: September 2015

No benefit to structured illumination microscopy for scattering imaging

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Super-resolution microscopy has received a lot of interest in the past few years, culminating in the 2014 Nobel Prize in Chemistry for the development of the STED and STORM/PALM family of techniques. Around the same time, an interesting (and mischievously titled) commentary appeared in Nature Photonics, claiming to resolve (ho ho) a misconception about a third approach to super-resolution – SIM or ‘structured illumination microscopy’. This is a technique which can be used to improve the resolution by a factor of two. The paper argues that structured illumination microscopy only provides true resolution enhancement for fluorescence imaging and none at all for scattering imaging. This is despite recent papers making claims – and apparently providing experimental evidence – to the contrary.
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